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Behavioral & Functional Phenotyping

Comprehensive Behavioral & Functional Package

Comprehensive behavioral and functional assessment across in vivo disease models

This matrix represents a comprehensive, multi-layered framework for behavioral and functional assessment in in vivo studies, designed to capture disease-relevant functional outcomes and therapeutic responses with high scientific rigor. Rather than relying on isolated tests, it integrates a systematic, decision-oriented set of behavioral, motor, cognitive, sensory, metabolic, and physiological readouts, directly aligned with disease mechanisms, drug classes, and preclinical study objectives. The categorization follows internationally accepted standards in neuroscience, pharmacology, and translational research, enabling targeted module selection based on the specific disease model, underlying biological pathways, and scientific questions of each project. The matrix is built to deliver interpretable, reproducible, and defensible data, supporting critical preclinical decisions including efficacy evaluation, safety assessment, go / no-go strategy, scientific publication, and regulatory-facing development pathways.

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    Veterinary Industry Innovation

    1

    General Activity, Locomotion & Exploratory Behavior

    Baseline activity levels, locomotion patterns, and exploratory responses

    Tests :

    • Open Field Test (OFT)
    • Home Cage Activity Monitoring
    • Locomotor Activity Box (Infrared Beam Break)
    • Rearing & Grooming Behavioral Scoring
    Veterinary Industry Innovation

    2

    Anxiety-Like Behavior

    Anxiety-related risk assessment and avoidance behavior

    Tests:

    • Elevated Plus Maze (EPM)
    • Elevated Zero Maze
    • Light–Dark Box Test
    • Open Field Center Time (Thigmotaxis Analysis)
    • Novelty-Suppressed Feeding (NSF) Test
    Veterinary Industry Innovation

    3

    Depression-Like Behavior & Motivation

    Affective state, motivation, and reward sensitivity

    Tests:

    • Forced Swim Test (FST)
    • Tail Suspension Test (TST)
    • Sucrose Preference Test
    • Novelty-Induced Hypophagia (NIH)
    Veterinary Industry Innovation

    4

    Learning, Memory & Cognition

    Spatial, associative, and recognition memory performance

    Tests:

    • Morris Water Maze (MWM)
    • Barnes Maze
    • Y-Maze Spontaneous Alternation
    • T-Maze (Alternation / Discrimination)
    • Radial Arm Maze (RAM)
    • Novel Object Recognition (NOR)
    • Object Location Memory Test
    • Passive Avoidance Test
    • Fear Conditioning (Contextual & Cued)
    Veterinary Industry Innovation

    5

    Motor Function, Strength & Coordination

    Motor control, balance, strength, and coordination

    Tests:

    • Rotarod Test
    • Balance Beam Test
    • Gait Analysis (CatWalk / Footprint Analysis)
    • Grip Strength Test
    • Wire Hang / Inverted Screen Test
    • Cylinder Test (Forelimb Use Asymmetry)
    • Ladder Rung Walking Test
    Veterinary Industry Innovation

    6

    Sensorimotor Integration & Reflexes

    Sensorimotor processing and reflex integrity

    Tests:

    • Startle Response & Prepulse Inhibition (PPI)
    • Righting Reflex
    • Placing / Stepping Reflexes
    • Adhesive Removal (Tape Removal) Test
    • Hot Plate Latency
    Veterinary Industry Innovation

    7

    Pain & Nociception

    Thermal, mechanical, inflammatory, and neuropathic pain responses

    Tests:

    • Hot Plate Test
    • Tail Flick Test
    • Von Frey Filament Test
    • Hargreaves Test
    • Formalin Test
    • Acetic Acid Writhing Test
    • Neuropathic Pain Behavioral Assays
      • Mechanical Allodynia
      • Thermal Hyperalgesia
      • Cold Allodynia
    Veterinary Industry Innovation

    8

    Seizure & Epilepsy-Related Behavioral Readouts

    Seizure susceptibility, severity, and temporal characteristics

    Tests:

    • Racine Seizure Scoring System
    • Latency to First Seizure
    • Seizure Frequency & Duration Monitoring
    • Video-EEG–Coupled Behavioral Scoring
    Veterinary Industry Innovation

    9

    Sleep & Circadian Rhythm

    Sleep architecture and circadian activity regulation

    Tests:

    • EEG/EMG-Based Sleep Scoring (NREM / REM / Wake)
    • Activity–Rest Cycle Monitoring (Actigraphy)
    • Sleep Latency Test
    • Sleep/Wake Fragmentation Analysis
    Veterinary Industry Innovation

    10

    Social Behavior & Communication

    Social interaction, dominance, and communication patterns

    Tests:

    • Three-Chamber Social Interaction Test
    • Social Approach / Social Avoidance Test
    • Resident–Intruder Test
    • Ultrasonic Vocalization (USV) Recording
    Veterinary Industry Innovation

    11

    Reward, Addiction & Motivation

    Reinforcement learning and substance-related behavior

    Tests:

    • Conditioned Place Preference (CPP)
    • Self-Administration (Operant Drug Intake)
    • Progressive Ratio Schedule
    • Operant Conditioning (Lever Press / Nose Poke)
    Veterinary Industry Innovation

    12

    Fatigue, Endurance & Exercise Capacity

    Physical endurance and fatigue resistance

    Tests:

    • Treadmill Exhaustion Test
    • Voluntary Wheel Running
    • Grip Strength Fatigue Protocols
    Veterinary Industry Innovation

    13

    Gastrointestinal & Feeding Behavior

    Feeding patterns, appetite regulation, and GI function

    Tests:

    • Food Intake Monitoring (24 h, Fast–Refeed)
    • Meal Pattern Analysis (Frequency / Size)
    • Conditioned Taste Aversion
    • GI Transit Test (Charcoal Meal / Dye)
    • Pica Behavior (Kaolin Intake)
    Veterinary Industry Innovation

    14

    Metabolic & Energy Balance Functional Readouts

    Energy expenditure, metabolism, and body composition

    Tests:

    • Indirect Calorimetry (VO₂, VCO₂, RER)
    • Integrated Activity, Food Intake & Energy Expenditure
    • Body Composition Analysis (DEXA / NMR)
    Veterinary Industry Innovation

    15

    Urinary & Bladder Function Behavioral Readouts

    Lower urinary tract function and voiding behavior

    Tests:

    • Voiding Spot Assay
    • Cystometry-Coupled Behavioral Assessment
    • Frequency–Volume Recording
    • Incontinence / Dribbling Scoring
    سنجش نقطه تخلیه ارزیابی رفتاری همراه با سیستومتری فرکانس – ضبط صدا بی اختیاری / امتیاز دهی دریبل
     
    Veterinary Industry Innovation

    16

    Cardiovascular & Stroke Functional Readouts

    Functional outcomes in cardiac and cerebrovascular models

    Tests:

    • Neurological Deficit Score (Stroke Models)
    • Post-Stroke Functional Battery (Rotarod / Cylinder / Ladder)
    • Treadmill Exercise Tolerance (HF / MI Models)
    • Telemetry-Based Activity with HR / BP Correlation
    Veterinary Industry Innovation

    17

    Wound Healing & Regenerative Functional Readouts

    Functional recovery following tissue injury and regeneration

    Tests:

    • Wound Closure Rate (Planimetry)
    • Limb Use Score (Ischemic Limb / Nerve Injury)
    • Post-Injury Gait Analysis
    • Functional Recovery Scores
    • Sciatic Functional Index (SFI)